PRKN and in situ carcinoma: In vivo experiments, both Western blot and immunohistochemistry analyses showed that the expression of PINK1 and Parkin in the kidney tissues of mice in the CIS-AKI group was lower than that in the control group (p < 0.05), and this reduction was significantly reversed (p < 0.01) following DM-AKG treatment (Fig. 7a–b).