To test whether IRF6 regulates glycolysis-mediated tumor progression by inhibiting PGM1 transcription in neuroblastoma, we constructed a PGM1 overexpression plasmid (Fig. 4A, B) and then cotransfected it with Flag-IRF6 and PGM1 plasmids or corresponding empty vectors for subsequent experiments in SK-N-BE2 and CHP-212 cells. The gene discussed is PGM1; the disease is neoplasm.