IRF6 and neuroblastoma: More importantly, we utilized RT‒qPCR and western blotting to measure IRF6 expression in fresh-frozen tissues from 4 with high-risk neuroblastoma patients and 4 with low-risk neuroblastoma patients, as well as in the intermediate-risk cell line SH-SY5Y and the low-risk cell line SK-N-SH, and the high-risk cell lines CHP-212 and SK-N-BE2 [19].