OPTN and glaucoma: Imaris-based 3D reconstruction and volume quantification revealed that expression of OPTN carrying the glaucoma-associated E50K mutation results in a significant increase in both the fraction of RGC axonal mitochondria outside of the LC3b-labeled RGC axons (13.4%) but also the fraction of all stopped RGC axonal mitochondria co-localizing with OPTN also outside of the LC3b-labeled RGC axons (17.9%), as compared to the much lower amounts found after expression of Wt OPTN (2.7% for mitochondria and 0.5% for mito-OPTN) (Figure 3D, E).