NCOA4 expression was downregulated in most glioma cell lines compared with astrocytes; it was significantly decreased in LN229, TJ905, A172, T98G, U251 and SNB19 cell lines and slightly decreased in 23N, U87, LN18 and B2-17 cell lines (Fig. 2A). This evidence concerns the gene NCOA4 and central nervous system cancer.