Of note, it is essential to highlight that the use of cytokines as biomarkers of MASLD may face some critical challenges in the clinical context: (a) many cytokines implicated in MASLD, such as IL-1β, TNF-α, and IL-6, are also involved in obesity, IR, and T2DM, which usually coexist with MASLD. This evidence concerns the gene TNF and obesity due to melanocortin 4 receptor deficiency.