Since a clinical application of NIR-PIT would be relatively easy for breast cancers, and MCF-7 would be a good model for cancer that can be treated with EpCAM-NIR-PIT but cannot be treated with EGFR-NIR-PIT, we used MCF-7 breast cancer as a model of EpCAM-targeted NIR-PIT in vivo. This evidence concerns the gene EGFR and breast cancer.