The other objectives were to evaluate whether combining PSA, ALP, DRE, and Gleason Score, with the additional parameters, cribriform pattern, perineural/perivascular invasion, percentage core involvement, and the symptom of bone pain improves the predictive accuracy for bone metastasis beyond PSA alone and to design a predictive model (risk estimator) out of it. This evidence concerns the gene KLK3 and bone metastasis.