TSC2 and tuberous sclerosis: We have previously utilized TSC patient induced pluripotent stem cell (iPSC)-derived neurons to show that dose-dependent reduction in TSC2 expression leads to dosage-sensitive cellular changes in neuronal morphology, including changes in gene expression, increased soma size and neurite outgrowth, as well as neuronal hyperexcitability (Winden et al., 2023; Winden et al., 2019).