The present study aimed to investigate the impact of T2D and GD on the intrauterine environment by measuring AMH, sex steroids, APN, insulin, and IGF-1 levels in the VCB of pregnancies with T2D and GD, compared to hormonal profile in VCB of pregnancies with normal glucose levels and to analyze the hormonal profiles of the corresponding mothers at the time of delivery. This evidence concerns the gene AMH and type 2 diabetes mellitus.