MAGEA1 and melanoma: To induce expression of MAGE genes in vitro by DAC treatment, we first tested different treatment regimens on the melanoma cell line SK2.3 that naturally expresses very low levels of MAGEA1. First, SK2.3 was treated on four consecutive days with different concentrations of DAC and analyzed for changes in MAGEA1 mRNA expression by qPCR (qPCR) (Figure 1A).