IHC staining of resection LTFs from 8 tumors representing 5 different tumor types showed reduced total cells (P<0.05, Figure S3A–B), reduced CD4+ cells (P < 0.05, Figure S3C) and a trend toward reduced CD8+ cells (P = 0.07, Figure S3D) after 48 hours of culture. This evidence concerns the gene CD4 and neoplasm.