While the magnitude of increase in IFNγ and CXCL10 in response to αCD3/αCD28 between cross-well and sequential treatment varied, the directionality of response was in high agreement with 5/6 tumor samples showing >1.5 fold change for IFNγ and all tumor samples showing >1.5 fold change for CXCL10 (Figure 5C). Here, CXCL10 is linked to neoplasm.