To assess whether PIEZO1 mediates NLGN3-induced ADAM10 activation, we conducted a CSPG4 shedding assay on glioma cells pre-treated with either the non-specific mechanosensitive channel blocker gadolinium ion (Gd3+) or the PIEZO1-specific blocker spider toxin GsMTx4 (Fig. 4e, Extended Data Fig.. 2d)50. Here, CSPG4 is linked to central nervous system cancer.