NLGN3 and glioblastoma: We then immobilized the NLGN3 ectodomains on commercial streptavidin resin (Fig. 1a and b) Next, we isolated and solubilized membranes from patient-derived cell cultures of DIPG/DMG or adult GBM (Fig. 1a) and passed each glioma membrane sample through the column in separate experiments.