Expanding beyond high-grade pediatric gliomas such as DIPG/DMG, we established that NLGN3-induced CSPG4 shedding occurs across a spectrum of glioma subtypes, including human IDH1 WT adult glioblastoma (Fig. 2c) and a mouse model of NF1-associated optic pathway glioma3 (Fig. 2d). Here, NLGN3 is linked to glioma.