At the earliest pre-clinical time point, Pik3ca deletion resulted in a decrease in the number or immunophenotypically defined LSCs (Figure 2C, Supplementary Figure 4D), defined as GFP+ granulocyte-macrophage progenitors for the KMT2A-MLLT3 AML model [33]. The gene discussed is PIK3CA; the disease is acute myeloid leukemia.