Considering that MMP9, E-cadherin, and Vimentin are linked to tumour invasion and metastasis — with Ki-67 serving as an indicator of cell proliferation — we verified their expressions in the pL-NC-LM3 and pL-sh-AC026412.33-LM3 groups via IHC and qRT-PCR analyses. The gene discussed is VIM; the disease is neoplasm.