GSEA employing the c2.all.v2022.1.Hs.symbols.gmt curated gene set further revealed that disulfidoptosis in hepatocellular carcinoma is fundamentally orchestrated by hepatic metabolic reprogramming, notably suppression of bile acid biosynthesis (e.g., CYP7A1/CYP27A1). The gene discussed is CYP27A1; the disease is hepatocellular carcinoma.