Four weeks after cell injection, tumor growth was significantly increased in mice with stable ACAT1 knockdown, whereas TRIM59 knockdown—either alone (shNC+siTRIM59) or combined with ACAT1 knockdown (shACAT1+siTRIM59)—led to a marked reduction in both tumor volume and weight compared to their respective controls (Fig. 7A–C). The gene discussed is TRIM59; the disease is neoplasm.