CRISPR-Cas9 depletion of ALG3 (sgALG3_2 and sgALG3_3) led to induction of ER stress in MDA-MB-468 breast cancer cells, as determined by increased protein (Fig. 3A) and mRNA (Fig. 3B) of the ER stress and UPR marker 78 kDA glucose-regulated protein/binding immunoglobulin protein (GRP78/BiP). Here, ALG3 is linked to breast cancer.