PIWIL2 and colonic neoplasm: Furthermore, examination of the methylation levels of the PIWIL2 promoter using UALCAN showed that this was significantly elevated in colon tumors overall, indicated by a higher methylation beta-value, as well as when these tumors were stratified by stage, nodal metastasis; p53 status; histological subtype; age; biological sex; weight; and race (Fig. 3).