In conclusion, the results of this study suggest that NCOA4 expression is closely related to the development of cholangiocarcinoma, and silencing NCOA4 expression can promote the proliferation, migration, and invasion of cholangiocarcinoma cells and is associated with the prognostic survival of patients with cholangiocarcinoma, and may play a role by reducing ferroptosis from occurring thus. Here, NCOA4 is linked to cholangiocarcinoma.