We also assessed Diap1 transcription in TOR-depleted BC clusters using the standard reporter Diap1-lacZ and observed its levels were significantly reduced compared to the control (mean intensity of Diap1-lacZ in control=558.7 A.U.; TORRNAi=354.8 A.U) (Fig. 4A-C) (Koontz et al., 2013; Yu and Pan, 2018). This evidence concerns the gene DIAPH1 and breast cancer.