In addition to the PI3K/AKT, Wnt/β-catenin and JAK/STAT signaling pathways, the NF-κB signaling pathway also has crosstalk mechanisms with several other signaling pathways, which play an important role in the occurrence and development of osteosarcoma and jointly regulate the biological behavior of osteosarcoma cells. The gene discussed is NFKB1; the disease is osteosarcoma.