ESR2 and hypospadias: A similar pattern was observed for ERβ in the prepuce, with strong expression in controls (2.8 ± 0.5), intermediate expression in distal (1.9 ± 0.7) and mid-penile hypospadias (1.5 ± 0.8), and weak expression in proximal hypospadias (0.6 ± 0.2).