Microglia survive in brain slices and can be studied using Iba1 or CD11b antibodies. The addition of lipopolysaccharide (LPS) stimulates microglia to migrate, and the addition of granulocyte macrophage colony-stimulating factor activates differentiation. Microglia can be damaged to mimic impaired function in AD. The gene discussed is AIF1; the disease is Alzheimer disease.