To explore the regulatory effect of miR-142-5p on PD-L1 protein expression, plasmids GV268, GV268-miR-142-5p, and synthetic oligonucleotides (miR mimic NC, miR-142-5p mimic, miR inhibitor NC, and miR-142-5p inhibitor) of equal concentrations were transfected into esophageal cancer cells. Here, CD274 is linked to esophageal cancer.