The highly increased lysosomal content is closely associated with membrane repair, as evidenced by the gradual increase in the intracellular expression of the lysosomal marker CD107a from the center to the periphery of melanoma tumor nodules in Sox10-high melanoma cells.95 Membrane repair also requires the involvement of ESCRT proteins.346 After perforin enters the target cell, ESCRT proteins are precisely recruited to the CTL contact site, where they participate in the repair of perforin pores and limit the entry of granzymes into the target cell. The gene discussed is PRF1; the disease is melanoma.