Using an unbiased screen, we cloned a high LS174T colonic adenocarcinoma MUC2 mucin-producing goblet-like cell that revealed by whole genome sequencing, mutations that upregulated the expression of tumor suppressor <i>TP53</i>, anterior gradient 2 (<i>AGR2</i>) and mitogen-activated protein kinase kinases (<i>MEK</i> and <i>MAPK</i>) genes that enhanced MUC2 biosynthesis and secretion. This evidence concerns the gene MUC5AC and colon adenocarcinoma.