In this work, following a thorough in silico assessment of G4 content in lncRNAs dysregulated in CRC, we first assessed the expression of LINC01589, MELTF-AS1, and UXT-AS1 in a series of CRC cells and then showed that G4s can fold within these lncRNAs both in vitro (via a series of established techniques including circular dichroism (CD), thermal difference spectra (TDS), fluorescence titrations, melting assays (FRET-MC) and mass spectrometry) and in CRC cells (via the G4-RNA-specific precipitation (G4RP) technique) [22, 53–55]. This evidence concerns the gene MELTF and colorectal carcinoma.