When comparing migraine patients without medication overuse and HC, NfL, and GFAP levels did not differ significantly (GFAP 71.38 pg/mL [IQR 54.86–108.08] vs. 69.43 pg/mL [IQR 53.04–8.99], p = 0.696; NfL 8.61 pg/mL IQR [7.08–10.88], p = 0.743). This evidence concerns the gene GFAP and migraine disorder.