The two genes, GIPC1 and RIPK2, where the two heterozygous splicing variants with isoform changes in K562 were found, of interest, are involved in tumor growth or invasion17,18 while the two genes, ULK3 and XRCC4, where the two heterozygous splicing variants with isoform changes in HepG2 were found, are known to be elevated in squamous cell carcinoma and associated with cancer susceptibility19,20. The gene discussed is ULK3; the disease is squamous cell carcinoma.