We constructed a mouse tumor model to investigate the impact of FAM111B silencing in vivo; employed tissue microarray to explore the prognostic value of different FAM111B expression levels; and applied western-blot assay, MYC-overexpression rescue experiments, protein transcriptomics, and bioinformatics analysis to examine the downstream molecular mechanisms underlying FAM111B actions. This evidence concerns the gene FAM111B and neoplasm.