Continuous bioluminescence imaging revealed a significant reduction in metastatic outgrowth in the lungs of mice injected with SPAT-overexpressing Calu1 cells over a period of 5 weeks (Fig. 2H and Supplementary Fig. S3H), Subsequent immunohistochemical (IHC) analyses of tumor tissues from nude mice revealed that SPAT overexpression decreased Ki67 and TWIST expression levels. This evidence concerns the gene MKI67 and neoplasm.