TWIST1 and neoplasm: Continuous bioluminescence imaging revealed a significant reduction in metastatic outgrowth in the lungs of mice injected with SPAT-overexpressing Calu1 cells over a period of 5 weeks (Fig. 2H and Supplementary Fig. S3H), Subsequent immunohistochemical (IHC) analyses of tumor tissues from nude mice revealed that SPAT overexpression decreased Ki67 and TWIST expression levels.