With the employment of markers for senescence cell (P21, γ-H2AX, and Lamin B1), GBM cells (GFAP), Macrophages (IBA1), and M2 macrophages (CD163), we observed that there were significantly more senescent TAMs (detected by P21, γ-H2AX and IBA1 positive, and Lamin B1 negative) than senescent GBM cells (P21, γ-H2AX, and GFAP positive, and Lamin B1 negative) in GBM tumors, and that there was a higher infiltration of M2 TAMs (CD163 positive) in GBM (Fig. 4A). This evidence concerns the gene AIF1 and glioblastoma.