Fig. 1 shows the location of the five residues selected for mutagenesis (highlighted in black) on the reported cryo-EM structures of tau fibrils isolated from deceased human patient samples neuropathologically diagnosed with (A) AGD, (B) CBD, (C) GGT, and (D) PSP. For comparison, the fibril structure of tau isolated from Tg2541+/+ mice, which express 0N4R human tau with the P301S mutation (Allen et al., 2002), is shown in Fig. 1E. All five cryo-EM structures were reported by Michel Goedert and Sjors Scheres (Schweighauser et al., 2023; Shi et al., 2021; Zhang et al., 2020). This evidence concerns the gene MAPT and argyrophilic grain disease.