Additionally, spatial multiplexed analysis using the MACSimaTM imaging platform and the MACS® iQ View–Spatial Biology software enabled the determination of the area covered by stroma, tumor, and CAR-target-expressing cells, which was identified by the expression of collagen (type I, III or IV), cytokeratin-7 and FOLR1, respectively (Fig 4A, B and C; Supporting Information S2 File). Here, FOLR1 is linked to neoplasm.