We strategically selected three cohorts of candidate TARDBP splicing regulators: (1) RBPs implicated as causative genes in ALS (TDP-43, FUS, and hnRNP A1) (Akçimen et al., 2023; Zhou et al., 2014), (2) those exclusively expressed in neurons (ELAV like RBP 3 [ELAVL3] and NOVA alternative splicing regulator 1 [NOVA1]) (Zhou et al., 2014), and (3) RBPs purportedly associated with ALS pathophysiology (hnRNP K, hnRNP E1, and hnRNP E2) (Bampton et al., 2021; Braems et al., 2022; Honda et al., 2023; Sidhu et al., 2022; Yoshimura et al., 2021). The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.