Both the probiotic (8.515 ± 2.751 ng/l) and myo-ins (13.51 ± 3.53 ng/l) treatments showed a (P < 0.05) reduction in the TNF-α levels compared to the PCOS group, with myo-ins demonstrating a more pronounced effect. The gene discussed is TNF; the disease is polycystic ovary syndrome.