To further investigate the effect of LGG on liver fibrosis in mice induced by HFD + CCl4, we examined the mRNA levels of profibrotic markers α-SMA, Collagen I, tissue inhibitor of metalloproteinase-1 (TIMP-1), and TGF-β and SMAD in the TGF-β/SMAD signaling pathway. The gene discussed is TIMP1; the disease is Hepatic fibrosis.