We used different knockout mice (TLR2–/–, TLR3–/–, TLR4–/–, TLR7–/–, TLR9–/–, MyD88–/–, STING–/–, cGAS–/–, IFNAR–/–, IL-1R–/–, caspase 1/11–/–, and gasdermin D–/–); we found that only MyD88 was essential for tumor regression and important for the induction of cellular infiltrate and inflammatory profile modulation in the TME in response to BCG intratumoral treatment. The gene discussed is TLR4; the disease is neoplasm.