Administration of DNP significantly reduced blood glucose, serum insulin, and HOMA-IR levels in PCOS rats, and restored the expression of IGF1 and IGF1R. Besides, DNP intervention can also promote GCs glycolysis and improve the mechanism of follicular dysplasia. Importantly, SIRT2 may be a key factor in regulating the glycolysis rate of granulosa cells by DNP. Here, SIRT2 is linked to polycystic ovary syndrome.