To investigate the correlation between the pathogenic WFS1 variants and clinical phenotypes, 75 variants classified as pathogenic from the ClinVar library (75 out of 93 pathogenic variants had recorded clinical phenotypes) were analyzed for the influence of clinical manifestations, such as DM, OA, DI, hearing impairment (HI), endocrine symptom (ES) other than DM, neurological symptoms (NSs), USs, PSs by covariates, including exons, domains, topology, ATP6VIA-interaction region, variant types and molecular consequence. The gene discussed is WFS1; the disease is diabetes mellitus.