CYP7A1 and hydrops fetalis: Interestingly, Cyp7a1 in LFVHC-fed mice at 8 weeks was significantly increased relative to LF, LFVHC, and HF-fed groups at week 24; however, no differences were observed among groups within the 24 week timepoint (Figure 4A, top panel), suggesting a transient impact of dietary cholesterol on BA synthesis genes.