To establish an ALS cellular model, we obtained Tet-on NEUROG2/ISL1/LHX3 iPSCs with TALLEN-mediated FUS_H517D mutations (Ichiyanagi et al., 2016) and treated them with doxycycline to induce differentiation (Supplementary Figure 1). Here, LHX3 is linked to amyotrophic lateral sclerosis.