To establish an ALS cellular model, we obtained Tet-on NEUROG2/ISL1/LHX3 iPSCs with TALLEN-mediated FUS_H517D mutations (Ichiyanagi et al., 2016) and treated them with doxycycline to induce differentiation (Supplementary Figure 1). The gene discussed is ISL1; the disease is amyotrophic lateral sclerosis.