TRPC1 and fragile X syndrome: To elucidate the predominant potential pathways engaged by TRPC1 in mediating mGluR-LTD, hippocampal brain slices from FXS C1 and FXS C1-cKO were stimulated with 100 μM DHPG for 0, 5, 60 min, and protein expression or phosphorylation was assessed later-on via western blot analysis (Figure 5A).