Furthermore, functional enrichment analysis, ssGSEA, and correlation analysis between the risk score and ssGSEA score corroborated that tumors with higher risk scores had higher activation of TGF-β signaling, hypoxia, EMT, angiogenesis, and stromal components, such as ECM, CAF, and MDSC, across pan-cancer. This evidence concerns the gene TGFB1 and cancer.