Using TGF-β1 treatment to induce EMT in eight ovarian cancer cell lines, we first sought to establish the relationship between TG2 and EMT in these cell lines by assessing the EMT genes CDH2, SNAI1, and VIM. TGF-β1 stimulated expression of at least one EMT marker in six of the cell lines and increased TGM2 in five of the cell lines (Figure 2B; Supplementary Figure S2A). Here, VIM is linked to ovarian cancer.