Based on histological features and immunostaining for SYTO13, PanCK, CD3D, and CD68, we selected eight regions of interest (ROIs) and defined segments representing tumor cells (SCC_Tumor, PanCK+, n = 8) and the surrounding TME (SCC_TME, PanCK‐, n = 8) (Figure 6A,B). The gene discussed is CD3D; the disease is neoplasm.