MGMT and glioblastoma: In the last years, various studies have been conducted on established and primary GBM cell lines, grown either in two-dimensional (2D) or 3D fashion, with the aim to highlight the key factors involved in metabolomics reprogramming correlated to malignant gliomas and GBM, also considering the presence of IDH gene mutation, the MGMT gene methylation status or the response to different treatments (Table 1).