INS and type 2 diabetes mellitus: Bottom-up data-dependent proteomic analysis (DDA—where individual peptide precursor ions are sequentially selected for fragmentation based on their intensity in survey spectrum) was extensively used for deep muscular proteome profiling, yielding novel data on fiber type specific skeletal muscle adaptations to exercise training23, convergent effects of acute exercise or insulin stimulation on muscular phosphoproteome24 or muscular protein–protein interactome in healthy and obese T2DM subjects25.