Among these 305 samples, TGFB1 appeared in 153 samples (50%), followed by EGFR in 148 samples (48.52%) at 2nd place and MAPK1 in 143 samples (46.88%), whereas in the case of DEGs, SPRR2G shows the highest LogFC value, followed by KRTDAP and KLK5. Bedi et al. in their study show that the elevated role of TGFB1 within the tumor microenvironment (TME) is associated with evading response to therapy and developing resistance45. This evidence concerns the gene MAPK1 and neoplasm.