Sal B inhibited the expression of BMP-2, p-Smad1, p-Smad5, Osterix, and Runx2 in a dose-dependent manner, demonstrating that Sal B may exert a protective effect on CKD-AVF rats by inhibiting BMP2/Smads/Runx2/Osterix signaling. This evidence concerns the gene PPIB and chronic kidney disease.