And even though we detected very low levels of HAVCR2 (encoding TIM3) by scRNA-seq, we confirmed the presence of TIM3-positive T cells in CLL LN tissue, and assumed an involvement of this interaction in immune escape as galectin-9 binding to TIM3 promotes CD4+ TREG development in CLL62. This evidence concerns the gene CD4 and B-cell chronic lymphocytic leukemia.